METABOLIC PROFILING OF HUMAN TUMOR CELLS BY ISOTOPOMER ANALYSIS AND 13C NMR SPECTROSCOPY

Abstract

This study concerns the analysis of human tumor cells metabolome HL60 and HL60-MX2 (Mitoxantrone resistant derivative of HL60 cell line displaying multidrug resistance) based on 13C NMR spectroscopy. Our aim was: i) the possible evaluation of the mechanisms of action of antitumor drugs, and ii) the identification of the metabolic processes inducing sensibility or resistance to a specific drug in relation with the peculiar metabolic phenotype. We analysed the metabolic profiles of acute myeloid leukaemia (AML) cell lines, HL60 and HL60-MX2, by means of 13C NMR mono and two-dimensional spectra, using [1,2-13C2]glucose as tracer for the isotopomer analysis. By comparing the analysis of key metabolites from the above lines it has been possible to describe and interpret the adaptation phenomena and the metabolic phenotypes of each cell line.