Cytotoxicity in vitro of two non-commercial samples of chrysotile and fibrous erionite

Authors

  • Armanda Pugnaloni Università Politecnica delle Marche
  • Silvia Silvia Di Valerio Department of Clinical and Molecular Sciences, Polytechnic University of Marche, Via Tronto 10/a, I-60126 Torrette di Ancona, Italy.
  • Tatiana Armeni Department of Clinical and Odontostomatological Sciences - Section Biochemistry, Biology and Physics, Polytechnic University of Marche.
  • Laura Cianfruglia Department of Clinical and Odontostomatological Sciences - Section Biochemistry, Biology and Physics, Polytechnic University of Marche.
  • Luca Tiano Department of Life and Environmental Sciences, Polytechnic University of Marche
  • Fabio Marchegiani
  • Pierluigi Stipa Science and Engineering of Materials, Environment and Urban Planning, Chemistry Division, Polytechnic University of Marche
  • Emiliano Laudadio Science and Engineering of Materials, Environment and Urban Planning, Chemistry Division, Polytechnic University of Marche
  • Simona Sabbatini Science and Engineering of Materials, Environment and Urban Planning, Chemistry Division, Polytechnic University of Marche
  • Cristina Minnelli Science and Engineering of Materials, Environment and Urban Planning, Chemistry Division, Polytechnic University of Marche
  • Salvatore Vaiasicca Department of Clinical and Molecular Sciences, Polytechnic University of Marche
  • Deborah Ramini Department of Clinical and Molecular Sciences, Polytechnic University of Marche
  • Antonio D. Procopio
  • Francesca Fazioli Department of Clinical and Molecular Sciences, Polytechnic University of Marche.
  • Alessandro F. Gualtieri Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia

DOI:

https://doi.org/10.13133/2239-1002/18017

Abstract

 

 

Exposure to naturally occurring asbestos and fibrous erionite can have severe impacts for the human health and cause malignant mesothelioma. The disease burden associated with this exposure is difficult to quantify and is not properly investigated. A characterization of the crystal-chemical-physical properties and toxicity/carcinogenicity of naturally occurring fibres with attention to asbestos and erionite is therefore highly recommended.

The present study deals with the in vitro biological effects in MeT5A and A549 human cell lines of two non-commercial asbestos fibres: Valmalenco chrysotile from Central Alps Italy and fibrous erionite from Jersey Nevada, USA, including the standards UICC Chrysotile and UICC crocidolite.

Light and scanning electron microscopy (SEM) observations showed morphological cell perturbation of treated cells. SEM-EDAX investigations revealed that the release of chemical components in the culture media affect cell viability. ROS levels increased in accordance with glutathione depletion, witnessing a remarkable cellular stress. Free radical release on pristine fibres was investigated by means of electron paramagnetic resonance (EPR) spin trapping technique and FTIR spectroscopy to characterize the molecular structures of fibres.
Results reflect the cytotoxic effects and evidence the different toxicity pathways prompted by these fibre species due to their different crystal assemblages and physical properties.

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Published

2023-08-01

Issue

Vol. 92 No. 2 (2023)

Section

TOXICITY AND CARCINOGENICITY OF MINERAL FIBRES: AN UPDATE. What have we learned after four years of PRIN? Guest Editors: Alessandro F. Gualtieri and Paolo Ballirano

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