Clinical Diagnosis and Cancer Probe: A History of Unity and Mass Migration

Abstract

In 1964 the mobility of Spanish biochemists from Europe to the United States resulted in a simple method to estimate the molecular weight of proteins (SDS-Page). Five years before, the idea that modifications of DNA-histones binding stopped RNA synthesis came at the hand of Vincent G. Allfrey in 1959. The discovery showed crucial and its interaction with the notable Spanish research program in US provided a new direction for the design of cancer diagnostic probes. In 1985, Manuel Perucho unified this view with the relevance of the polymerase that synthesizes DNA and RNA, in a method to detect single point mutations in oncogenic genes. It is expected to expose here the history of this useful approach who sought to use histone modifications in cancer clinical practice.