We report the case of an anencephalic foetus petrified by Gerolamo Segato in the course of his experiments on body conservation. The specimen has been studied applying non-invasive methods. Digital radiography and computed tomography (CT) alogside more advanced techniques such as three-dimensional (3-D) reconstruction and virtual endoscopy (VE) have been used to investigate anatomic morphology and to perform hypotheses about Segato’s method of petrification which is still unknown.