First PCR isolation of Crithidia mellificae (Euglenozoa: Trypanosomatidae) in Apis mellifera (Hymenoptera: Apidae) in Italy

Authors

  • Antonella Cersini Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Rome
  • Valeria Antognetti Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Rome
  • Raffaella Conti Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Rome
  • Fabiana Velletrani Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Rome
  • Giovanni Formato Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Rome

DOI:

https://doi.org/10.13133/2284-4880/133

Keywords:

Crithidia mellificae, honey bee, Italy

Abstract

Crithidia mellificae (Langridge & McGhee, 1967) is a trypanosomatid described in Apis mellifera (Linnaeus, 1758). The pathological effects of this parasite on the host are not well known. In this short communication, we report the first isolation of this pathogen in Italy, as realized in December 2013. The detection of Crithidia spp. was obtained by applying two PCR protocols that target the sequence of the mitochondrial cytochrome b (Cytb) and the sequence of small subunit ribosomal RNA (SSUrRNA), respectively. The PCR products were subjected to sequencing, which confirmed that the strain belonged to Crithidia mellificae.

Downloads

Download data is not yet available.

Downloads

Published

2015-06-30

How to Cite

Cersini, A., Antognetti, V., Conti, R., Velletrani, F. and Formato, G. (2015) “First PCR isolation of Crithidia mellificae (Euglenozoa: Trypanosomatidae) in Apis mellifera (Hymenoptera: Apidae) in Italy”, Fragmenta entomologica, 47(1), pp. 45–49. doi: 10.13133/2284-4880/133.

Issue

Vol. 47 No. 1 (2015)

Section

Short Scientific Notes