Gravity constraint in cell phenotypic determination
AbstractDistinct phenotypes emerge spontaneously when mammalian cells are cultured under microgravity conditions. Such finding is explained by the interplay among the intrinsic stochasticity, which, in turn, is successively ‘canalized’ and sustained by the activation of a specific gene regulatory network. However, when the two cell subsets are reseeded into a normal gravity field the two phenotypes collapse into one. Gravity constraints the system in adopting only one phenotype. Cell fate commitment is achieved through a de novo reshaping of the overall cell morphological and functional organization, and cannot be explained as a ‘selecting’ effect. Those findings highlight how constraints – acting as global order factors – drive cell specification and behavior. These data cast on doubt the current explanatory bottom-up, molecular based models.
How to Cite
Copyright Agreement with Authors
Before publication, after the acceptance of the manuscript, authors have to sign a Publication Agreement with Organisms. The authors retain all rights to the original work without any restrictions.
License for Published Contents
You are free to copy, distribute and transmit the work, and to adapt the work. You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work).